WB result of GHR Recombinant Rabbit mAb
Primary antibody: GHR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 71 kDa
This blot was developed with high sensitivity substrate
GHR Recombinant Rabbit mAb (S-1320-123)
GHR Recombinant Rabbit mAb (S-1320-123)
Price:
Regular price
$130.00 SGD
Regular price
Sale price
$130.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GHR |
| Synonyms | Growth hormone receptor, GH receptor, Somatotropin receptor |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane |
| Accession | P10912 |
| Clone Number | S-1320-123 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| ICC | 1:50 | null |
Background
The growth hormone receptor (GHR) is a cell surface receptor that binds to growth hormone (GH), a hormone responsible for stimulating growth, cell reproduction, and cell regeneration in humans. When GH binds to GHR, it triggers a cascade of intracellular signaling events, including the activation of JAK2/STAT, MAPK, and PI3K pathways, which play crucial roles in growth and development. GHR is highly expressed in liver cells but also found in other tissues such as the kidneys, skeletal muscle, and the central nervous system. Mutations or alterations in the GHR can lead to growth abnormalities and have been implicated in conditions like Laron syndrome, which is characterized by dwarfism and high levels of GH due to GHR insensitivity. Understanding the structure and function of GHR is vital for developing therapeutic strategies for growth-related disorders.
Picture
Picture
Western Blot
WB result of GHR Recombinant Rabbit mAb
Primary antibody: GHR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 71 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-GHR antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
