WB result of GFP Recombinant Rabbit mAb (HRP Conjugate) (S-296-32)
Lysate: Aequorea victoria GFP Protein 20 ng
Primary antibody: GFP Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | GFP |
Synonyms | Green fluorescent protein |
Immunogen | Synthetic Peptide |
Accession | P42212 |
Clone Number | S-296-32 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | WB |
Reactivity | Species Independent |
Purification | Protein A |
Concentration | 2 mg/ml |
Conjugation | HRP |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
application | dilution | species |
WB | 1:5000-1:50000 |
Background
The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. [PMID: 28749] The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.
Picture
Picture
Western Blot
WB result of GFP Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: GFP Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: untreated 293T whole cell lysate 2 µg
Lane 2: 293T transfected with GFP tag whole cell Lysate 2 µg
Predicted MW: 28 kDa
Observed MW: 28 kDa
