WB result of GDF-15 Recombinant Rabbit mAb
Primary antibody: GDF-15 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HT-1080 whole cell lysate 20 µg
Lane 2: HT-1080 treated with 200 ng/ml TPA for 24 hours whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 34 kDa
GDF-15 Recombinant Rabbit mAb (S-1012-42)
GDF-15 Recombinant Rabbit mAb (S-1012-42)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GDF-15 |
| Synonyms | Growth/differentiation factor 15, Macrophage inhibitory cytokine 1 (MIC-1), NSAID-activated gene 1 protein (NAG-1), NSAID-regulated gene 1 protein (NRG-1), Placental TGF-beta, Placental bone morphogenetic protein, Prostate differentiation factor, MIC1, PDF, PLAB, PTGFB |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | Q99988 |
| Clone Number | S-1012-42 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IP | 1:50 | null |
| IHC-P | 1:500 | null |
Background
Growth differentiation factor-15 (GDF-15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a member of the transforming growth factor-β (TGF-β) superfamily. It is a stress-responsive cytokine that is produced by various cell types including macrophages, vascular smooth muscle cells, cardiomyocytes, adipocytes, and endothelial cells. GDF-15 is typically expressed at low levels in most tissues but can be significantly induced under conditions of tissue injury, hypoxia, and inflammatory cytokine responses. It plays a role in various biological processes such as organ growth, differentiation, development, and cellular repair. Notably, GDF-15 has been identified as a biomarker for disease prognosis, particularly in cardiovascular diseases, cancer, and metabolic disorders. The cytokine has also been implicated in immune modulation and has been proposed as a potential target for therapeutic intervention in conditions like obesity, diabetes, and cardiovascular diseases. Its role as an immune checkpoint and its potential as a target for cancer immunotherapy are areas of active research.
Picture
Picture
Western Blot
IP
GDF-15 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating GDF-15 in 0.4 mg LNCaP whole cell lysate.
Western blot was performed on the immunoprecipitate using GDF-15 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: LNCaP whole cell lysate 20 µg (Input)
Lane 2: GDF-15 Rabbit mAb IP in LNCaP whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in LNCaP whole cell lysate
Predicted MW: 34 kDa
Observed MW: 34 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti- GDF-15 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti- GDF-15 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti- GDF-15 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
