WB result of GAPDH Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: GAPDH Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Predicted MW: 37 kDa
Observed MW: 37 kDa
GAPDH Recombinant Rabbit mAb (HRP Conjugate) (S-240-147)
GAPDH Recombinant Rabbit mAb (HRP Conjugate) (S-240-147)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GAPDH |
| Synonyms | Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Membrane |
| Accession | P04406、 P16858 |
| Clone Number | S-240-147 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Zf |
| Positive Sample | HeLa, MCF7, NIH/3T3, mouse brain,C6, rat brain, zebrafish |
| Predicted Reactivity | Hm, Mi, Pg, Sq, Dg, Ct, Sh, Qu, Bv, Ck |
| Purification | Protein A |
| Concentration | Lot specific* (generally 0.8 to 1 mg/ml)* |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | Hu, Ms, Rt |
Background
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH comprises a polypeptide chain of 335 amino acids. Structural studies identified two regions, namely the glyceraldehyde-3-phosphate catalytic site and the NAD+ binding site, a primary structure known as the Rossmann fold, which is also required for the activity of other dehydrogenases. Many of these roles are dependent on the ability of GAPDH to bind different macromolecules in the cell.
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Western Blot
WB result of GAPDH Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: GAPDH Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Predicted MW: 37 kDa
Observed MW: 37 kDa
WB result of GAPDH Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: GAPDH Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Predicted MW: 37 kDa
Observed MW: 37 kDa
