WB result of GABRD Recombinant Rabbit mAb
Primary antibody: GABRD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse kidney lysate 20 µg
Lane 2: unboiled mouse brain lysate 20 µg
Lane 3: unboiled mouse cerebellum lysate 20 µg
Negative control: unboiled mouse kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51 kDa
Observed MW: 51 kDa
GABRD Recombinant Rabbit mAb (S-1584-162)
GABRD Recombinant Rabbit mAb (S-1584-162)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GABRD |
| Synonyms | Gamma-aminobutyric acid receptor subunit delta; GABA(A) receptor subunit delta (GABAAR subunit delta) |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P22933 |
| Clone Number | S-1584-162 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse brain, mouse cerebellum, rat brain, rat cerebellum |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:200-1:500 | Hu, Ms, Rt |
Background
GABRD is the delta subunit of the GABA (Gamma-Aminobutyric Acid) A-type receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain, and its action on GABA-A receptors can be modulated by various drugs such as barbiturates, benzodiazepines, steroids, or alcohol. The role of GABRD in various cancers has been studied. For instance, in Colon Adenocarcinoma (COAD), the expression of GABRD is significantly elevated and associated with advanced clinical staging. High GABRD expression correlates with shorter overall survival and progression-free survival in patients, making it an independent predictor of overall survival. In Breast Cancer, GABRD promotes breast cancer progression through CDK1-dependent cell cycle regulation, with significantly elevated expression levels in tumor tissues and correlation with advanced tumor progression. Additionally, GABRD is considered prognostic in Bladder Urothelial Carcinoma and Clear Cell Renal Cell Carcinoma. In Gastric Cancer (GC), high expression levels of GABRD mRNA are associated with poor prognosis. The function of GABRD in proliferation, invasion, and apoptosis experiments has been assessed, indicating that GABRD plays a significant role in GC cell proliferation and invasion and is correlated with poor prognosis, suggesting that GABRD may be a potential therapeutic target for gastric cancer.
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Western Blot
WB result of GABRD Recombinant Rabbit mAb
Primary antibody: GABRD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat kidney lysate 20 µg
Lane 2: unboiled rat brain lysate 20 µg
Lane 3: unboiled rat cerebellum lysate 20 µg
Negative control: unboiled rat kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51 kDa
Observed MW: 51 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-GABRD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-GABRD antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GABRD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GABRD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-GABRD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
