WB result of FXR1 Rabbit pAb
Primary antibody: FXR1 Rabbit pAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDa
FXR1 Rabbit Polyclonal Antibody
FXR1 Rabbit Polyclonal Antibody
Price:
Regular price
$131.00 SGD
Regular price
Sale price
$131.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FXR1 |
| Synonyms | RNA-binding protein FXR1; FMR1 autosomal homolog 1; hFXR1p |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P51114 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | MCF7, HEK-293, HeLa, HepG2, A549, C2C12, mouse liver, mouse skeletal muscle, rat liver, rat skeletal muscle |
| Predicted Reactivity | Bv, Hm |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
FXR1 (Fragile X-related protein 1) is an evolutionarily conserved RNA-binding protein that contains three KH domains and an RGG box, enabling it to recognize and regulate the stability, splicing, subcellular transport and translation of a wide spectrum of mRNAs and miRNAs; it shuttles between nucleus and cytoplasm, acts as a cytoplasmic m⁶A reader, forms functional amyloid aggregates in neurons, controls muscle and vascular smooth-muscle cytoskeleton organization, blood pressure and inflammatory transcript decay, and is indispensable for early embryogenesis, placental trophoblast migration, neuronal miRNA processing and long-term memory, while its over-expression or amplification is linked to poor prognosis in multiple cancers.
Picture
Picture
Western Blot
WB result of FXR1 Rabbit pAb
Primary antibody: FXR1 Rabbit pAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70-90 kDa
WB result of FXR1 Rabbit pAb
Primary antibody: FXR1 Rabbit pAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 90 kDa
WB result of FXR1 Rabbit pAb
Primary antibody: FXR1 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-FXR1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
