WB result of FXR Recombinant Rabbit mAb
Primary antibody: FXR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: MDA-MB-231 whole cell lysate 20 µg
Lane 5: A431 whole cell lysate 20 µg
Lane 6: HeLa whole cell lysate 20 µg
Lane 7: A549 whole cell lysate 20 µg
Lane 8: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 56 kDa
FXR Recombinant Rabbit mAb (S-2845-71)
FXR Recombinant Rabbit mAb (S-2845-71)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FXR |
| Synonyms | Bile acid receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14 (RXR-interacting protein 14); BAR; HRR1; RIP14; NR1H4 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q96RI1 |
| Clone Number | S-2845-71 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | 293T, HepG2, MCF7, MDA-MB-231, A431, HeLa, A549, HCT 116 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
The Farnesoid X receptor (FXR, NR1H4) is a ligand-activated nuclear receptor that functions as the master bile-acid sensor: after binding physiological bile acids such as chenodeoxycholic acid, FXR heterodimerizes with RXR, translocates to DNA FXR-response elements and trans-activates or represses genes that control bile-acid synthesis (CYP7A1), conjugation (BACS, BAAT), export pumps (BSEP, MRP2) and intestinal re-uptake (IBABP, ASBT), while additionally regulating lipid and glucose metabolism, hepatic lipogenesis, energy balance, inflammatory signaling, gut barrier integrity and innate immunity; FXR exists as four human isoforms (α1–α4) and is expressed mainly in liver, intestine and kidney, with synthetic agonists (e.g. GW4064, obeticholic acid) and antagonists under intense investigation for treating cholestasis, NASH, fibrosis, diabetes and intestinal inflammatory disorders.
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Western Blot
