WB result of FUNDC1 Rabbit pAb
Primary antibody: FUNDC1 Rabbit pAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Negative control: THP-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
FUNDC1 Rabbit Polyclonal Antibody
FUNDC1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FUNDC1 |
| Synonyms | FUN14 domain-containing protein 1 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion outer membrane |
| Accession | Q8IVP5 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, T-47D, MCF7, RAW264.7, mouse brain, C6, rat brain |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200-1:500 | Hu, Ms, Rt |
Background
The FUNDC1 protein, also known as FUN14 domain containing 1, is a mitochondrial protein that plays a critical role in mitochondrial dynamics and cellular respiration. It is involved in various metabolic pathways, including fatty acid metabolism, glycolysis, and oxidative phosphorylation. FUNDC1 is highly expressed in tissues such as the brain and blood, and it is enriched in immune cells like monocytes and neutrophils. The protein is also implicated in immune responses and has been associated with processes such as antigen presentation and the regulation of immune cell functions. Additionally, FUNDC1 has been linked to the detoxification of reactive oxygen species (ROS) and is involved in the metabolism of several vitamins and amino acids.
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Western Blot
WB result of FUNDC1 Rabbit pAb
Primary antibody: FUNDC1 Rabbit pAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
WB result of FUNDC1 Rabbit pAb
Primary antibody: FUNDC1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
