WB result of FOXP2 Recombinant Rabbit mAb
Primary antibody: FOXP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 70 kDa
FOXP2 Recombinant Rabbit mAb (S-2342-8)
FOXP2 Recombinant Rabbit mAb (S-2342-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FOXP2 |
| Synonyms | Forkhead box protein P2; CAG repeat protein 44; Trinucleotide repeat-containing gene 10 protein; CAGH44; TNRC10 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | O15409 |
| Clone Number | S-2342-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | HEK-293, HeLa, HCT 116, Neuro-2a |
| Predicted Reactivity | NHPr, Xe |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms |
| ICC | 1:100 | Hu |
Background
FOXP2 protein is a transcription factor that plays a crucial role in the development and functioning of certain neural circuits related to language and speech production. It is encoded by the FOXP2 gene. Mutations in this gene can lead to speech and language disorders. The protein is expressed in various regions of the brain, including the basal ganglia and the cortex. It helps regulate the expression of other genes involved in the formation of neural connections and the development of motor skills necessary for speech. FOXP2 is also found in other species and is believed to be involved in vocalizations and other forms of communication. Its discovery and study have provided valuable insights into the genetic basis of language and the complex mechanisms underlying human communication abilities.
Picture
Picture
Western Blot
WB result of FOXP2 Recombinant Rabbit mAb
Primary antibody: FOXP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- FOXP2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
