WB result of FMRP Recombinant Rabbit mAb
Primary antibody: FMRP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: A375 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 71 kDa
Observed MW: 75 kDa
FMRP Recombinant Rabbit mAb (S-1671-1)
FMRP Recombinant Rabbit mAb (S-1671-1)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FMRP |
| Synonyms | Fragile X messenger ribonucleoprotein 1; Fragile X messenger ribonucleoprotein; Protein FMR-1; FMR1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q06787 |
| Clone Number | S-1671-1 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, 293T, HeLa, A431, A375, C2C12, NIH/3T3, PC-12 |
| Predicted Reactivity | Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
FMRP, or Fragile X Mental Retardation Protein, is a multifunctional RNA-binding protein that plays a crucial role in synaptic development and plasticity in the nervous system. It is widely expressed across all tissues, although at varying levels, and is not restricted to the synapse or neurons. It is involved in regulating the fate of stem cells, being essential for stem cell maintenance and inhibiting stem cell differentiation. FMRP binds to a variety of RNAs, including methylated histone, ribosome, mRNP, and other proteins. It is known to interact with a specific set of mRNAs, aiding in their transport along microtubules and regulating their translation through association with ribosomes. FMRP is established as a translation repressor, but recent studies on mouse and human FXS models suggest that FMRP is involved in regulating almost all aspects of gene expression. It can also enhance the translation of its target mRNAs by binding to the coding sequence and hindering ribosome movement on mRNA. FMRP deficiency is associated with Fragile X Syndrome (FXS), the most common inherited cause of intellectual disability and autism. FMRP has also been implicated in cancer growth and immune evasion.
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Western Blot
WB result of FMRP Recombinant Rabbit mAb
Primary antibody: FMRP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 71 kDa
Observed MW: 75 kDa
WB result of FMRP Recombinant Rabbit mAb
Primary antibody: FMRP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 71 kDa
Observed MW: 75 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
