WB result of Flotillin 1 Rabbit pAb
Primary antibody: Flotillin 1 Rabbit pAb at 1/1000 dilution
Lane 1: SK-OV-3 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
Flotillin 1 Rabbit Polyclonal Antibody
Flotillin 1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Flotillin 1 |
| Synonyms | FLOT1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | O75955 |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | SK-OV-3, 293T, A431, C2C12, C6, PC-12 |
| Predicted Reactivity | Mq, Or, Pg, Bv |
| Purification | Protein A |
| Concentration | Lot specific* (generally 0.2 to 2 mg/ml)* |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
Background
Flotillin-1 is a lipid raft marker protein that is involved in a variety of cellular physiological activities and is associated with the development of many types of cancer. In terms of cellular functions, Flotillin-1 helps maintain cell membrane structure, participates in signal transduction, is involved in endocytosis, and plays a role in cell adhesion. It interacts with various signaling molecules to form signaling complexes that regulate intracellular signaling pathways. For example, it associates with Src kinase and is involved in processes such as cell growth, differentiation, and apoptosis. Flotillin-1 has also been implicated in the pathology of several diseases. It is highly expressed in lung adenocarcinoma, breast cancer, and hepatocellular carcinoma, and its expression is associated with poor prognosis and low overall survival rates. Flotillin-1 is involved in the activation of signaling pathways, such as the IGF-1 receptor, and it plays a role in the intracellular localization and signaling function of this receptor. Moreover, Flotillin-1 has been shown to be essential for the efficient sorting of T cell receptors into Rab11a vesicles for fusion with the plasma membrane, indicating its role in immune cell function. In terms of neurological diseases, Flotillin-1 has been linked to neurodegenerative conditions such as Parkinson's disease, where its expression levels are significantly elevated in the substantia nigra/ventral tegmental area.
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Western Blot
WB result of Flotillin 1 Rabbit pAb
Primary antibody: Flotillin 1 Rabbit pAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
WB result of Flotillin 1 Rabbit pAb
Primary antibody: Flotillin 1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression Atlas
Expression of Flotillin 1 in tumor tissue
Expression of Flotillin 1 in human tissue
Expression of Flotillin 1 in mouse & rat tissue
