Flow cytometric analysis of Mouse Ly-6C expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Brilliant Violet 510™ Rat Anti-Mouse CD11b Antibody and either FITC Rat IgG2c, κ Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse Ly-6C Antibody (Right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Mouse Ly-6C Antibody (S-R523)
FITC Rat Anti-Mouse Ly-6C Antibody (S-R523)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ly-6C |
| Synonyms | Lymphocyte antigen 6C1; Ly-6C1; Ly6c1 |
| Location | Cell membrane |
| Accession | P0CW02 |
| Clone Number | S-R523 |
| Antibody Type | Rat mAb |
| Isotype | IgG2c,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer |
50 mM Na-citrate, pH 6.0, 0.9 % NaCl, 1% BSA, 0.3% Proclin 300
|
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen belonging to the Ly-6 family of glycosyl phosphatidylinositol-anchored proteins. It is primarily expressed on monocytes/macrophages, endothelial cells, neutrophils, and certain subsets of T cells, including memory CD8+ T cells. Ly-6C is regulated by interferon gamma and plays a role in the development and maturation of lymphocytes. In mice, Ly-6C+ monocytes are a major subset of circulating monocytes known for their phagocytic activity and inflammatory functions, while Ly-6C- monocytes exhibit patrolling behavior along blood vessels. This marker is also used to differentiate between various immune cell populations in research settings.
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