Flow cytometric analysis of Mouse CD90.2 (Thy-1.2) expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD3 Antibody and either FITC Rat IgG2a, κ Isotype Control (Left panel) or SDT FITC Rat anti-Mouse CD90.2 (Thy-1.2) Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Mouse CD90.2 (Thy-1.2) Antibody (S-R546)
FITC Rat Anti-Mouse CD90.2 (Thy-1.2) Antibody (S-R546)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD90.2 (Thy-1.2) |
| Synonyms | Thy-1 membrane glycoprotein; Thy-1 antigen; CD90; Thy-1; Thy1 |
| Location | Cell membrane |
| Accession | P01831 |
| Clone Number | S-R546 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD90.2 (Thy-1.2) is a 25-35 kDa glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein and a member of the immunoglobulin superfamily. It is primarily expressed on hematopoietic stem cells, neurons, all thymocytes, and peripheral T cells in mouse strains such as BALB/c, C57BL/6, and C3H. This molecule plays a role in cell adhesion, signal transduction, and the regulation of T cell activation. Additionally, CD90.2 is involved in lymphocyte proliferation and hematopoietic stem cell differentiation. It is also notable for its interaction with CD45 and its potential role in cell-cell interactions during synaptogenesis in the brain.
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