Flow cytometric analysis of Mouse CD14 expression on RAW 264.7 Cells from the RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) or Neuro-2a (Mouse neuroblastoma neuroblast, Left) was stained with FITC Rat IgG2a, κ Isotype Control (Black line histogram) and SDT FITC Rat Anti-Mouse CD14 Antibody (Red line histogram) at 0.25 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Mouse CD14 Antibody (S-R609)
FITC Rat Anti-Mouse CD14 Antibody (S-R609)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse CD14 |
| Synonyms | Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein |
| Location | Cell membrane |
| Accession | P10810 |
| Clone Number | S-R609 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | RAW 264.7 |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Ms |
Background
CD14, also known as the LPS receptor, is a surface antigen belonging to the family of cell surface glycoproteins. It exists in two forms: membrane-bound CD14 (mCD14), which is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) tail, and soluble CD14 (sCD14), found in the plasma. CD14 is primarily expressed on monocytes, macrophages, and dendritic cells, and plays a critical role in the immune system by recognizing and binding to lipopolysaccharide (LPS) from Gram-negative bacteria, leading to cellular activation and the initiation of inflammatory responses.
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