Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling SDT FITC Mouse IgM, κ Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT CD13 antibody at 1 μg/test (S0B0441) (Right) / (Red line histogram), Mouse monoclonal IgG Isotype Control (Goat Anti - Mouse IgG FITC was used as the secondary antibody) (Left) / (Black line histogram) compared with SDT FITC Mouse IgM, κ Isotype Control Antibody (Right) / (Black line histogram) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse IgM, κ Isotype Control Antibody (S-844-28)
FITC Mouse IgM, κ Isotype Control Antibody (S-844-28)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Clone Number | S-844-28 |
| Antibody Type | Mouse mAb |
| Application | FCM |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling SDT FITC Mouse IgM, κ Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with α-tubulin antibody (Right) / (Red line histogram), Mouse monoclonal IgG Isotype Control (Goat Anti - Mouse IgG FITC was used as the secondary antibody) (Left) / (Black line histogram) compared with SDT FITC Mouse IgM, κ Isotype Control Antibody (Right) / (Black line histogram) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
