Flow cytometric analysis of Human CD22 expression on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 Antibody and either Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human Siglec-2/CD22 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Human Siglec-2/CD22 Antibody (S-565-1)
FITC Mouse Anti-Human Siglec-2/CD22 Antibody (S-565-1)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Siglec-2/CD22 |
| Synonyms | B-cell receptor CD22; B-lymphocyte cell adhesion molecule (BL-CAM); Sialic acid-binding Ig-like lectin 2 (Siglec-2); T-cell surface antigen Leu-14; SIGLEC2 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P20273 |
| Clone Number | S-565-1 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood lymphocytes |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu |
Background
Siglec-2, also known as CD22, is a type I transmembrane protein belonging to the immunoglobulin superfamily and functions as an inhibitory lectin on B cells. It is characterized by its ability to bind α2,6-linked sialic acid on the same (cis) or adjacent (trans) cells. CD22 plays a crucial role in mediating B cell interactions, directing B cell localization within lymphoid tissues, and regulating B cell antigen receptor signaling. The protein contains four immunoreceptor tyrosine-based inhibitory motifs (ITIMs), allowing it to recruit cytoplasmic phosphatases to inhibit downstream signaling pathways. Additionally, CD22 can form cis interactions with sialylated glycoproteins such as CD45, potentially masking its sialic acid recognition site. Its complex interactions with various signaling molecules highlight its multifaceted role in B cell regulation and immune responses.
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