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FITC Mouse Anti-Human CD8 Antibody (S-R533)

FITC Mouse Anti-Human CD8 Antibody (S-R533)

Catalog Number: S0B5007 Application: FCM Reactivity: Human Conjugation: FITC Brand: Starter
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Regular price $228.00 SGD
Regular price Sale price $228.00 SGD
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Product Details

Product Specification


Host Mouse
Antigen CD8
Synonyms T-lymphocyte differentiation antigen T8/Leu-2; T-cell surface glycoprotein CD8 alpha chain; MAL; CD8a
Location Cell membrane
Accession P01732
Clone Number S-R533
Antibody Type Mouse mAb
Isotype IgG1
Application FCM
Reactivity Hu
Positive Sample Human PBMC
Purification Protein G
Concentration 0.05mg/ml
Conjugation FITC
Physical Appearance Liquid
Storage Buffer

PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Hu

Background

CD8A encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Picture

FC

Flow cytometric analysis of Human CD8 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either FITC Mouse IgG1 Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD8 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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