Flow cytometric analysis of Human CD7 expression on human peripheral blood. Human peripheral blood was stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either FITC mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD7 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
Host | Mouse |
Antigen | CD7 |
Synonyms | T-cell antigen CD7; GP40; T-cell leukemia antigen; T-cell surface antigen Leu-9; TP41 |
Immunogen | Recombinant Protein |
Location | Cell membrane |
Accession | P09564 |
Clone Number | S-575-17 |
Antibody Type | Mouse mAb |
Isotype | IgG1,k |
Application | FCM |
Reactivity | Hu |
Positive Sample | human peripheral blood |
Purification | Protein G |
Concentration | 0.05mg/ml |
Conjugation | FITC |
Physical Appearance | Liquid |
Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
application | dilution | species |
FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD7 is a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. CD7 can be aberrantly expressed in refractory anaemia with excess blasts (RAEB) and may confer a worse prognosis. Also, a lack of CD7 expression could insinuate mycosis fungoides (MF) or Sezary syndrome (SS).
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