| Multiparameter flow cytometric analysis of Human CD3d expression on human peripheral blood mononuclear cell populations. Human peripheral blood mononuclear cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 antibody and either FITC Mouse IgG2a Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD3d Antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software. |
FITC Mouse Anti-Human CD3d Antibody (S-R565)
FITC Mouse Anti-Human CD3d Antibody (S-R565)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD3d |
| Synonyms | T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; T3D |
| Location | Cell membrane |
| Accession | P04234 |
| Clone Number | S-R565 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu |
Background
CD3d, or the CD3 delta subunit of the T-cell receptor complex, is a crucial component of the T-cell receptor (TCR) complex. The TCR/CD3 complex, including CD3d, is essential for signal transduction in T-cells. When the TCR recognizes and binds to an antigen presented by MHC molecules, the CD3 complex, including CD3d, transduces the activation signal into the T-cell. Defects in the CD3d can lead to severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK), highlighting the importance of CD3d in the immune system.
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