Multiparameter flow cytometric analysis of Human CD127 expression on human peripheral blood mononuclear cell populations. Human peripheral blood mononuclear cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 antibody and either FITC Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD127 antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Human CD127 Antibody (S-R462)
FITC Mouse Anti-Human CD127 Antibody (S-R462)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD127 |
| Synonyms | Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127 |
| Location | Cell membrane |
| Accession | P16871 |
| Clone Number | S-R462 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu |
Background
CD127, also known as the interleukin-7 receptor alpha chain (IL-7Rα) or simply the IL-7 receptor, is a transmembrane glycoprotein primarily expressed on the surface of T cells, B cells, natural killer (NK) cells, as well as certain hematopoietic stem cells and progenitor cells. Upon binding to IL-7, CD127 activates a series of signaling pathways, including the JAK-STAT cascade, which subsequently promotes the growth and differentiation of lymphocytes. This interaction is particularly crucial during the development of T and B cells within the thymus and bone marrow, as it aids in their progression from early progenitor stages to maturity. Furthermore, the expression level of CD127 serves as an indicator for assessing lymphocyte function and status.
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