WB result of FbpB/Ag85B Recombinant Rabbit mAb
Primary antibody: FbpB/Ag85B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: fbpC protein 10 ng
Lane 2: fbpA protein 10 ng
Lane 3: fbpB protein 10 ng
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 40 kDa
This antibody does not cross-react with fbpA and fbpC
FbpB/Ag85B Recombinant Rabbit mAb (S-3325-22)
FbpB/Ag85B Recombinant Rabbit mAb (S-3325-22)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FbpB/Ag85B |
| Synonyms | Diacylglycerol acyltransferase/mycolyltransferase Ag85B; DGAT; 30 kDa extracellular protein; Acyl-CoA:diacylglycerol acyltransferase; Antigen 85 complex B (85B; Ag85B); Extracellular alpha-antigen; Fibronectin-binding protein B (Fbps B); fbpB |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P9WQP0 |
| Clone Number | S-3325-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Mycobacterium tuberculosis |
Background
FbpB/Ag85B is a 30 kDa secreted fibronectin-binding mycolyltransferase that catalyzes the transfer of mycolic acids to arabinogalactan and trehalose monomycolates, thereby building trehalose dimycolate (cord factor) and maintaining the integrity of the mycobacterial cell wall, while simultaneously functioning as the immunodominant antigen of the antigen 85 complex that strongly induces Th1-biased cellular and humoral immunity through IFN-γ, IL-2 and antibody production, making it a key adhesin for host-cell attachment and a leading subunit vaccine candidate against tuberculosis.
Picture
Picture
Western Blot
