WB result of F4/80 Recombinant Rat mAb
Primary antibody: F4/80 Recombinant Rat mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 130-300 kDa
F4/80 Recombinant Rat mAb (S-942-1)
F4/80 Recombinant Rat mAb (S-942-1)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | F4/80 |
| Synonyms | Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; Gpf480; Adgre1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q61549 |
| Clone Number | S-942-1 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,λ |
| Application | WB, IHC-P, FCM |
| Reactivity | Ms |
| Positive Sample | RAW264.7, mouse spleen, BALB/c mouse peritoneal exudates cells |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:1000 | Ms |
| FCM | 1:2000 | Ms |
Background
F4/80, also known as ADGRE1 or EMR1, is a cell surface glycoprotein and a member of the EGF-TM7 protein family. It is widely used as a marker for mature mouse macrophages and is highly expressed in various macrophage populations, such as Kupffer cells in the liver, Langerhans cells in the skin, and microglial cells in the brain. However, its expression in human cells is different, being mainly found in eosinophils and some macrophages. F4/80 is an orphan receptor involved in cell adhesion and immune cell interactions, and it may also play a role in the development of regulatory T cells. The protein has a large extracellular domain containing multiple EGF-like calcium-binding domains, linked to a seven-transmembrane domain characteristic of G protein-coupled receptors.
Picture
Picture
Western Blot
FC
Flow cytometric analysis of BALB/c mouse peritoneal exudates cells labelling F4/80 antibody at 1/2000 dilution (0.1 μg) / (Right panel) compared with a Rat IgG1, λ Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD11b - Brilliant Violet 421™ Antibody separately.
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-F4/80 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-F4/80 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cardiac muscle. Anti-F4/80 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
