WB result of Ezrin Recombinant Rabbit mAb
Primary antibody: Ezrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 80 kDa
Ezrin Recombinant Rabbit mAb (S-1894-4)
Ezrin Recombinant Rabbit mAb (S-1894-4)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Ezrin |
| Synonyms | Cytovillin; Villin-2; p81; VIL2; EZR |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cytoskeleton, Cell membrane |
| Accession | P15311 |
| Clone Number | S-1894-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Raji, NIH/3T3, C6 |
| Predicted Reactivity | Rb, Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
Ezrin is a key member of the Ezrin-Radixin-Moesin (ERM) protein family, functioning as a linker protein that connects the plasma membrane to the actin cytoskeleton. It plays a crucial role in mediating membrane-cytoskeleton interactions, regulating cell morphology, adhesion, migration, and survival. Structurally, Ezrin consists of three main domains: an N-terminal FERM domain that binds to membrane proteins and lipids, a central helical linker region, and a C-terminal ERM-associated domain (C-ERMAD) that interacts with actin filaments. In its inactive state, Ezrin is held in the cytoplasm through intramolecular interactions, but it can be activated by phosphorylation at Thr567, leading to its translocation to the plasma membrane. Ezrin is essential for various cellular functions, including the formation of membrane protrusions like microvilli and invadopodia, which are critical for cell motility and invasion. Abnormal regulation of Ezrin has been implicated in several diseases, particularly cancer, where its overexpression or dysregulation can promote tumor progression, invasion, and metastasis.
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Picture
Western Blot
WB result of Ezrin Recombinant Rabbit mAb
Primary antibody: Ezrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 80 kDa
WB result of Ezrin Recombinant Rabbit mAb
Primary antibody: Ezrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 80 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
