WB result of EphA2 Recombinant Rabbit mAb
Primary antibody: EphA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SK-MEL-28 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: U-87 MG whole cell lysate 20 µg
Lane 6: HeLa whole cell lysate 20 µg
Low expression control: PC-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 120 kDa
EphA2 Recombinant Rabbit mAb (S-2978-41)
EphA2 Recombinant Rabbit mAb (S-2978-41)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | EphA2 |
| Synonyms | Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; ECK; EPHA2 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P29317 |
| Clone Number | S-2978-41 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | K562, PC-3, U-87 MG, HeLa, NIH/3T3, L-929, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
Phospho-EphA2 (Tyr588) is the EphA2 receptor tyrosine kinase autophosphorylated on Tyr588, a modification that stabilizes the active kinase conformation, amplifies downstream Ras/MAPK, PI3K/Akt, RhoA and Src signaling cascades governing cell–cell repulsion, migration, invasion, anchorage-independent growth and angiogenesis, and thereby serves as a biomarker and potential therapeutic target in aggressive cancers.
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Western Blot
WB result of EphA2 Recombinant Rabbit mAb
Primary antibody: EphA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: L-929 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 120 kDa
WB result of EphA2 Recombinant Rabbit mAb
Primary antibody: EphA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 120 kDa
WB result of EphA2 Recombinant Rabbit mAb
Primary antibody: EphA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 120 kDa
