WB result of elF4A1 Rabbit pAb
Primary antibody: elF4A1 Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDa
elF4A1 Rabbit Polyclonal Antibody
elF4A1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | elF4A1 |
| Synonyms | Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1; DDX2A; EIF4A |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P60842 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, HeLa, Jurkat, A431, NIH/3T3, mouse liver, PC-12, C6 |
| Predicted Reactivity | CyMk, Or, Rb |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| ICC | 1:500 | Hu |
Background
eIF4A1, or eukaryotic translation initiation factor 4A1, is a protein that plays a crucial role in the initiation of translation in eukaryotic cells. It is one of the subunits of the eIF4F complex, which is responsible for unwinding the secondary structure of the mRNA 5' untranslated region (5' UTR) to facilitate the binding of the 40S ribosomal subunit to the start codon. Deregulated translation initiation, including the overactivity of eIF4A1, is implicated in cancer initiation and progression. This makes eIF4A1 an attractive target for cancer therapeutics. Researchers are actively pursuing strategies that target eIF4A1 to inhibit tumor growth and proliferation. Several natural product molecules, such as Rocaglamide A (RocA), have been reported to target eIF4A1, but their clinical utilization has been limited due to pharmacological limitations. In the context of cancer, eIF4A1 has been associated with poor outcomes in diffuse large B cell lymphoma (DLBCL). Higher expression levels of eIF4A1 are linked to shorter overall survival and progression-free survival in patients with DLBCL. This suggests that eIF4A1 expression could serve as a prognostic biomarker for this type of cancer. eIF4A1 is also involved in the translation of specific mRNAs that have complex secondary structures or are under translational control by factors such as the mTOR pathway. During mTORC1 inhibition, eIF4A1 enhances the translational repression mediated by LARP1 (La-related protein 1), which is a key regulator of mRNA translation.
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Western Blot
WB result of elF4A1 Rabbit pAb
Primary antibody: elF4A1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDa
WB result of elF4A1 Rabbit pAb
Primary antibody: elF4A1 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDa
IP
elF4A1 Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating elF4A1 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using elF4A1 Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: elF4A1 Rabbit pAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 46 kDa
Observed MW: 46 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- elF4A1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
