WB result of eIF3B Rabbit mAb
Primary antibody: eIF3B Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 120 kDa
(This blot was developed with high sensitivity substrate)
eIF3B Recombinant Rabbit mAb (S-816-8)
eIF3B Recombinant Rabbit mAb (S-816-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | eIF3B |
| Synonyms | Eukaryotic translation initiation factor 3 subunit B, Eukaryotic translation initiation factor 3 subunit 9, Prt1 homolog (hPrt1), eIF-3-eta, eIF3 p110, eIF3 p116, EIF3S9 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P55884 |
| Clone Number | S-816-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:200 |
Background
Translation initiation factor 3 (eIF3), the largest, most complex translation initiation factor, is involved in almost the entire process of translation initiation regulation, including ribosomal recruitment of the ternary complex, attachment of 43S complexes to mRNA via interaction with eIF4G, selection of the initiation codon (AUG), and prevention of premature association of the 40 and 60 S ribosomal subunits.
Picture
Picture
Western Blot
WB result of eIF3B Rabbit mAb
Primary antibody: eIF3B Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 120 kDa
(This blot was developed with high sensitivity substrate)
WB result of eIF3B Rabbit mAb
Primary antibody: eIF3B Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 120 kDa
(This blot was developed with high sensitivity substrate)
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
