WB result of eIF2A Recombinant Rabbit mAb
Primary antibody: eIF2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65 kDa
eIF2A Recombinant Rabbit mAb
eIF2A Recombinant Rabbit mAb
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | eIF2A |
| Synonyms | CDA02; EIF-2A; MST089; MSTP004; MSTP089 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q9BY44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | K562, LnCap, NIH3T3, PC12 |
| Purification | Protein A |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | |
| ICC | 1:50-1:200 |
Background
eIF2A (eukaryotic translation initiation factor 2A) is a regulatory protein involved in the initiation of protein translation, primarily functioning as an alternative translation initiation factor under stress conditions. Unlike the canonical eIF2, eIF2A does not rely on GTP or the loading of initiator tRNA. Instead, under specific conditions such as endoplasmic reticulum stress, amino acid starvation, or viral infection, it mediates non-canonical translation initiation, facilitating the translation of specific mRNAs, such as stress-related proteins or viral RNAs. The function of eIF2A is closely linked to cellular stress responses, viral infections, and tumorigenesis, although its precise mechanisms and regulatory networks require further investigation. The activity of eIF2A may provide potential therapeutic targets for certain diseases, such as cancer and neurodegenerative disorders.
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Picture
Western Blot
WB result of eIF2A Recombinant Rabbit mAb
Primary antibody: eIF2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65 kDa
WB result of eIF2A Recombinant Rabbit mAb
Primary antibody: eIF2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65 kDa
Immunocytochemistry
Immunocytochemistry analysis of eIF2A (green) in MCF-7 using eIF2A antibody,and DAPI(blue)
