WB result of ECM1 Recombinant Rabbit mAb
Primary antibody: ECM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 48, 85 kDa
ECM1 Recombinant Rabbit mAb (S-2235-35)
ECM1 Recombinant Rabbit mAb (S-2235-35)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ECM1 |
| Synonyms | Extracellular matrix protein 1; Secretory component p85; Ecm1 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | Q61508 |
| Clone Number | S-2235-35 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Ms |
| Positive Sample | C2C12, NIH/3T3, mouse liver |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| ICC | 1:500 | Ms |
Background
ECM1 protein, or extracellular matrix protein 1, is a glycoprotein predominantly expressed in the extracellular matrix of various tissues. It plays a crucial role in cell adhesion, proliferation, and differentiation. ECM1 is highly expressed in tissues such as skin, placenta, and blood vessels. Its expression is regulated by several growth factors and hormones. Abnormal expression of ECM1 has been implicated in various pathological conditions, including cancer progression, where it may contribute to tumor growth and metastasis by modulating the tumor microenvironment. Additionally, ECM1 is involved in the development and maintenance of normal tissue architecture and has potential as a biomarker for certain diseases due to its specific expression patterns.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti- ECM1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
