WB result of Dynamin 2 Recombinant Rabbit mAb
Primary antibody: Dynamin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 105 kDa
Dynamin 2 Recombinant Rabbit mAb (S-1751-209)
Dynamin 2 Recombinant Rabbit mAb (S-1751-209)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Dynamin 2 |
| Synonyms | Dynamin-2; Dynamin II; DYN2; DNM2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Endosome, Synapse |
| Accession | P50570 |
| Clone Number | S-1751-209 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, HeLa, K562, SH-SY5Y, Neuro-2a, mouse testis, PC-12, rat brain |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
Dynamin 2 is a large GTPase protein that plays a crucial role in cellular processes such as endocytosis and cytokinesis. It is involved in the fission of membrane vesicles, helping to separate newly formed vesicles from the parent membrane during endocytosis. This protein is widely expressed in various tissues and is essential for maintaining cellular homeostasis. Mutations in the dynamin 2 gene have been associated with several diseases, including centronuclear myopathy, a disorder characterized by muscle weakness and abnormal muscle structure. Its functions are highly conserved across species, highlighting its importance in fundamental cellular mechanisms.
Picture
Picture
Western Blot
WB result of Dynamin 2 Recombinant Rabbit mAb
Primary antibody: Dynamin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 105 kDa
WB result of Dynamin 2 Recombinant Rabbit mAb
Primary antibody: Dynamin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 105 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-Dynamin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Dynamin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-Dynamin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Dynamin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Dynamin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
