WB result of DR5 Rabbit mAb
Primary antibody: DR5 Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Low expression control: HeLa whole cell lysate
Predicted MW: 48 kDa
Observed MW: 40, 48 kDa
DR5 Recombinant Rabbit mAb (S-649-36)
DR5 Recombinant Rabbit mAb (S-649-36)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DR5 |
| Synonyms | Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL receptor 2; TRAIL-R2), CD262, TNFRSF10B, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9 |
| Immunogen | Synthetic Peptide |
| Location | Membrane |
| Accession | O14763 |
| Clone Number | S-649-36 |
| Antibody Type | Recombinant mAb |
| Application | WB, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 |
Background
DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Mice have a homologous gene, tnfrsf10b, that has been essential in the elucidation of the function of this gene in humans. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
Picture
Picture
Western Blot
IP
DR5 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating DR5 in 0.4 mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using DR5 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HepG2 whole cell lysate 20 µg (Input)
Lane 2: DR5 Rabbit mAb IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 48 kDa
Observed MW: 40, 48 kDa
