WB result of DNA Ligase IV/LIG4 Recombinant Rabbit mAb
Primary antibody: DNA Ligase IV/LIG4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 104 kDa
Observed MW: 100 kDa
DNA Ligase IV/LIG4 Recombinant Rabbit mAb (S-2237-118)
DNA Ligase IV/LIG4 Recombinant Rabbit mAb (S-2237-118)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DNA Ligase IV/LIG4 |
| Synonyms | DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | P49917 |
| Clone Number | S-2237-118 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | MCF7, HeLa, NIH/3T3, mouse testis, PC-12, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu |
| ICC | 1:1000 | Hu, Ms |
Background
DNA Ligase IV, also known as LIG4, is a crucial enzyme involved in the non-homologous end joining (NHEJ) pathway, which is essential for repairing DNA double-strand breaks. It plays a vital role in maintaining genomic stability by facilitating the ligation of broken DNA ends. LIG4 forms a complex with other proteins such as XRCC4 and XLF, enhancing its stability and activity. This enzyme is particularly important in the repair of DNA damage caused by ionizing radiation and certain chemotherapeutic agents. Mutations or deficiencies in LIG4 can lead to severe immunodeficiency and developmental disorders due to impaired V(D)J recombination, a process critical for the generation of antigen receptor diversity in the immune system.
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Western Blot
WB result of DNA Ligase IV/LIG4 Recombinant Rabbit mAb
Primary antibody: DNA Ligase IV/LIG4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 104 kDa
Observed MW: 100 kDa
WB result of DNA Ligase IV/LIG4 Recombinant Rabbit mAb
Primary antibody: DNA Ligase IV/LIG4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 104 kDa
Observed MW: 100 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-DNA Ligase IV/LIG4 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-DNA Ligase IV/LIG4 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
