WB result of DMC1 Recombinant Rabbit mAb
Primary antibody: DMC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 38 kDa
DMC1 Recombinant Rabbit mAb (S-2166-46)
DMC1 Recombinant Rabbit mAb (S-2166-46)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DMC1 |
| Synonyms | Meiotic recombination protein DMC1/LIM15 homolog; DMC1H; LIM15 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q14565 |
| Clone Number | S-2166-46 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Raji, K562, Molt-4, F-9, mouse testis, PC-12, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Ms, Rt |
Background
DMC1, a meiosis-specific RecA-homologous recombinase conserved from fungi to humans, is indispensable for accurate homologous chromosome segregation during meiosis: it loads onto the single-stranded DNA tails generated at programmed DNA-double-strand breaks, forms octameric rings that encircle the DNA, and drives the search for and strand invasion of homologous chromatids, thereby catalyzing the crossover events that stabilize bivalents; in so doing it works alongside (and partly suppresses) the ubiquitous RAD51, and its absence or mutation causes meiotic arrest, defective synaptonemal-complex formation, and sterility .
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Western Blot
WB result of DMC1 Recombinant Rabbit mAb
Primary antibody: DMC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 38 kDa
WB result of DMC1 Recombinant Rabbit mAb
Primary antibody: DMC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 38 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse testis. Anti-DMC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-DMC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-DMC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat liver. Anti-DMC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
