WB result of Cytokeratin 7 Rabbit mAb
Primary antibody: Cytokeratin 7 Rabbit mAb at 1/500 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: T47D whole cell lysate 20 µg
Lane 3: Hela whole cell lysate 20 µg
Lane 4: SK-OV-3 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 40~55 kDa
Cytokeratin 7 Recombinant Rabbit mAb (SDT-R028)
Cytokeratin 7 Recombinant Rabbit mAb (SDT-R028)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cytokeratin 7 |
| Synonyms | Cytokeratin-7 (CK-7); Keratin-7 (K7); Sarcolectin; Type-II keratin Kb7; KRT7 |
| Location | Cell membrane, Intracellular |
| Accession | P08729 |
| Clone Number | SDT-R028 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP, ICFCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | |
| IP | 1:25 | |
| IHC | 1:1000 | |
| ICC | 1:50 | |
| ICFCM | 1:500 |
Background
Cytokeratin 7 is the intermediate silk protein mainly expressed in epithelial cells. The heterodine of keratin consists of a polymerization filament by an acid -keratin (or type I keratin, keratin 9 to 23) and alkaline keratin (or type II keratin, keratin 1 to 8). Expression similar to but more limited than keratins 8 and 18 in simple, pseudostratified and ductal epithelium, mesothelium and urothelium. It's generally expressed (with some variation) in adenocarcinoma of lung, breast, thyroid, endometrium, cervix, ovary, salivary gland, upper GI tract, urothelial carcinoma, papillary renal cell carcinoma and Paget disease. It's generally negative (with some variation) in colorectal carcinoma, Merkel cell carcinoma, hepatocellular carcinoma, prostatic adenocarcinoma, adrenocortical tumors and squamous cell carcinoma.
Picture
Picture
Western Blot
FC
Flow cytometric analysis of MCF7 (left) / HeLa (right) cells labelling Cytokeratin-7 antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: MCF7
IP
Cytokeratin 7 Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating Cytokeratin 7 in 0.4mg T-47D whole cell lysate.
Western blot was performed on the immunoprecipitate using Cytokeratin 7 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: T-47D whole cell lysate 20µg(input)
Lane 2: Cytokeratin 7 Rabbit mAb IP in T-47D whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in T-47D whole cell lysate
Predicted MW: 52 kDa
Observed MW: 40-55 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer.
Anti-Cytokeratin 7 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer.
Anti-Cytokeratin 7 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embeddedhuman pancreas cancer.
Anti-Cytokeratin 7 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta.
Anti-Cytokeratin 7 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney.
Anti-Cytokeratin 7 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver.
Anti-Cytokeratin 7 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control.IHC shows negative staining in paraffin-embedded human colon.
Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
