WB result of CYP11A1 Rabbit pAb
Primary antibody: CYP11A1 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Negative control: mouse brain lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
CYP11A1 Rabbit Polyclonal Antibody
CYP11A1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CYP11A1 |
| Synonyms | Cholesterol side-chain cleavage enzyme, mitochondrial; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc); CYP11A |
| Immunogen | Synthetic Peptide |
| Accession | P05108 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse testis, rat testis |
| Predicted Reactivity | CyMk |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:200-1:1000 | Hu, Rt |
| IF | 1:500 | Hu |
Background
CYP11A1 (Cytochrome P450 Family 11 Subfamily A Member 1) is an enzyme belonging to the cytochrome P450 superfamily. It catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in steroid hormone synthesis. This reaction is essential for the production of glucocorticoids (e.g., cortisol), mineralocorticoids (e.g., aldosterone), and sex hormones. CYP11A1 is primarily expressed in the adrenal cortex, placenta, and gonads, where it plays a critical role in maintaining normal physiological functions and stress responses. Abnormal regulation of CYP11A1 has been implicated in various diseases, including adrenal hyperplasia, adrenocortical carcinoma, polycystic ovary syndrome (PCOS), and hormonal imbalances. For instance, artemisinin derivatives have been shown to inhibit CYP11A1 expression, reducing androgen synthesis and demonstrating potential therapeutic effects in PCOS. Additionally, CYP11A1 inhibitors, such as MK-5684, are being tested in clinical trials for prostate cancer, showing promising efficacy and tolerability. Overall, research on CYP11A1 is crucial for understanding the regulation of steroid hormone synthesis and provides a valuable target for developing novel therapies for hormone-related diseases.
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Western Blot
WB result of CYP11A1 Rabbit pAb
Primary antibody: CYP11A1 Rabbit pAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti-CYP11A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-CYP11A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-CYP11A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cardiac muscle. Anti-CYP11A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-CYP11A1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cardiac muscle. Anti- CYP11A1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human testis. Anti- CYP11A1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
