WB result of CRBN Recombinant Rabbit mAb
Primary antibody: CRBN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SK-MEL-28 whole cell lysate 20 µg
Lane 2: U266B1 whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Low expression control: SK-MEL-28 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 54 kDa
CRBN Recombinant Rabbit mAb (S-2044-2)
CRBN Recombinant Rabbit mAb (S-2044-2)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CRBN |
| Synonyms | Protein cereblon |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q96SW2 |
| Clone Number | S-2044-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | U266B1, HCT 116, PC-3, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Cereblon (CRBN), encoded by the CRBN gene located on chromosome 3p26.2, is an evolutionarily conserved 442-amino-acid substrate receptor of the CRL4A E3 ubiquitin ligase complex, which ubiquitinates target proteins for proteasomal degradation, thereby influencing developmental processes (e.g., limb and auditory vesicle formation), regulating potassium channels, and modulating immune functions, including T-cell proliferation and activation. CRBN also serves as the primary molecular target for thalidomide and its immunomodulatory drug derivatives (lenalidomide, pomalidomide), which inhibit CRBN autoubiquitination and modulate ubiquitination of downstream substrates, thereby exerting anti-myeloma and teratogenic effects. Mutations or dysregulation of CRBN are linked to autosomal recessive nonsyndromic mental retardation and may affect cell metabolism and disease development.
Picture
Picture
Western Blot
WB result of CRBN Recombinant Rabbit mAb
Primary antibody: CRBN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
WB result of CRBN Recombinant Rabbit mAb
Primary antibody: CRBN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
