WB result of COX1/Cyclooxygenase 1 Recombinant Rabbit mAb
Primary antibody: COX1/Cyclooxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HaCaT whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 69 kDa
Observed MW: 58, 70 kDa
COX1/Cyclooxygenase 1 Recombinant Rabbit mAb (S-1478-48)
COX1/Cyclooxygenase 1 Recombinant Rabbit mAb (S-1478-48)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | COX1/Cyclooxygenase 1 |
| Synonyms | Prostaglandin G/H synthase 1; Prostaglandin H2 synthase 1 (PGH synthase 1; PGHS-1; PHS 1); Prostaglandin-endoperoxide synthase 1; PTGS1 |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum membrane |
| Accession | P23219 |
| Clone Number | S-1478-48 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, HeLa, HaCaT, C2C12, NIH/3T3, mouse cerebellum, mouse kidney, rat cerebellum, rat kidney |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
COX1, full name Cyclooxygenase-1, is one of the two isoforms of cyclooxygenase (COX), with the other being COX-2. COX1 is a constitutive enzyme that is widely expressed in most tissues such as blood vessels, stomach, kidneys, and platelets. It is involved in platelet aggregation, vascular tone, gastric mucosal blood flow, and regulation of renal blood flow, helping to maintain the stability of cellular, tissue, and organ physiological functions. Compared to COX-2, COX1 is constitutively expressed and plays a role in the synthesis of physiological prostaglandins (PGs), which regulate normal tissue cell activities such as protecting the gastrointestinal mucosa and altering vascular tone. COX1 plays a smaller role in inflammatory damage compared to COX-2. Inflammation primarily stimulates monocytes, macrophages, etc., inducing the production of COX-2, which is a key link in triggering subsequent inflammatory responses.
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Western Blot
WB result of COX1/Cyclooxygenase 1 Recombinant Rabbit mAb
Primary antibody: COX1/Cyclooxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse cerebellum lysate 20 µg
Lane 4: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 69 kDa
Observed MW: 58, 70 kDa
WB result of COX1/Cyclooxygenase 1 Recombinant Rabbit mAb
Primary antibody: COX1/Cyclooxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat cerebellum lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 69 kDa
Observed MW: 56, 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human lung. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
