WB result of CDK1 Recombinant Rabbit mAb
Primary antibody: CDK1 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDa
CDK1 Recombinant Rabbit mAb (S-1374-66)
CDK1 Recombinant Rabbit mAb (S-1374-66)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CDK1 |
| Synonyms | Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDC2; CDC28A; CDKN1; P34CDC2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P06493 |
| Clone Number | S-1374-66 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, MCF7, HT-29, NIH/3T3, mouse testis, C6, rat testis |
| Predicted Reactivity | Bv, Ck, Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:2000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
CDK1, or Cyclin-dependent kinase 1, is a pivotal protein kinase that plays a critical role in controlling cell cycle progression. CDK1, in complex with cyclins (primarily cyclin B), is crucial for the progression of the cell cycle, particularly for the transition from G2 phase to mitosis (M phase). It helps in the initiation of mitosis by phosphorylating various substrates that are involved in chromosome condensation, nuclear envelope breakdown, and spindle assembly. CDK1 is involved in the regulation of transcription factors and chromatin modifiers, thereby influencing gene expression. It can modulate the activity of transcription factors like RUNX2, SIRT1/2, NPM1, and SREBF1, which mediate cell proliferation and apoptosis by modulating differentiation and metabolic processes. CDK1 plays a complex role in apoptosis. It can inhibit apoptosis through the phosphorylation of caspase-9 and BIRC5 proteins, but it can also promote cell death by phosphorylating and activating Bcl-2 family members. CDK1 regulates mitochondrial dynamics through the phosphorylation of proteins involved in mitochondrial fusion and fission. It also contributes to maintaining cellular redox balance and protects cells from oxidative stress.
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Western Blot
WB result of CDK1 Recombinant Rabbit mAb
Primary antibody: CDK1 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDa
WB result of CDK1 Recombinant Rabbit mAb
Primary antibody: CDK1 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDa
IP
CDK1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CDK1 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using CDK1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: CDK1 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 34 kDa
Observed MW: 34 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- CDK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- CDK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
