ICC shows positive staining in Jurkat cells. Anti- CD99 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
CD99 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-R122)
CD99 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-R122)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD99 |
| Synonyms | 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2 |
| Location | Membrane |
| Accession | P14209 |
| Clone Number | S-R122 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2.5 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:200 | null |
| IF | 1:200 | null |
Background
CD99 antigen (Cluster of differentiation 99), also known as MIC2 or single-chain type-1 glycoprotein, is a heavily O-glycosylated transmembrane protein that is encoded by the CD99 gene in humans. It is expressed on all leukocytes but highest on thymocytes and is believed to augment T-cell adhesion and apoptosis of double positive T cells. It has been found in endothelial cells and in the periodontium, including gingival fibroblasts and gingival epithelial cells. It also participates in migration and activation. It is found on the cell surface of Ewing's sarcoma tumors and is positive in granulosa cell tumors. It is more expressed in malignant gliomas than in the brain, and such overexpression results in higher levels of invasiveness and lower rates of survival. Antibodies to CD99 are used in diagnostic immunohistochemistry to distinguish Ewing's sarcoma from other tumours of similar histological appearance, as well as for the identification of thymic tumours, and of spindle cell tumours, such as synovial sarcoma, haemangiopericytoma, and meningioma.
Picture
Picture
Immunocytochemistry
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD99 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human Ewing's sarcoma. Anti-CD99 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human pancreas. Anti-CD99 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
