IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD81 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
CD81 Recombinant Rabbit mAb (SDT-144-22)
CD81 Recombinant Rabbit mAb (SDT-144-22)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD81 |
| Synonyms | CD81 antigen, TAPA-1, Tspan-28 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P60033 |
| Clone Number | SDT-144-22 |
| Antibody Type | Rabbit mAb |
| Application | IHC-P |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:200 | null |
Background
CD81 (encoded by the CD81 gene) deficiency is a human autosomal recessive disorder also resulting in hypogammaglobulinemia. CD81 is a tetraspanin molecule expressed on immune cells, hepatocytes, and most stromal and epithelial cells, unlike CD19 and CD21 which are mostly expressed on B cells.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-CD81 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD81 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CD81 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD81 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CD81 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
