Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD8α antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD4 - Alexa Fluor® 488 separately. Gated on total viable cells.
CD8α Recombinant Mouse mAb (S-1036-21)
CD8α Recombinant Mouse mAb (S-1036-21)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD8α |
| Synonyms | T-lymphocyte differentiation antigen T8/Leu-2; T-cell surface glycoprotein CD8 alpha chain; MAL |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P01732 |
| Clone Number | S-1036-21 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | IHC-P, FCM, IF |
| Reactivity | Hu |
| Positive Sample | Human tonsil, Human spleen, Human PBMC |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:200 | Hu |
| IF | 1:100 | Hu |
| FCM | 1:2000 | Hu |
Background
CD8A encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD8α antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD8α antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD8α antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
