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CD45RO Recombinant Mouse mAb (S-R401)

CD45RO Recombinant Mouse mAb (S-R401)

Catalog Number: S0B0705 Application: WB,IHC-P,FCM,IF Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Mouse
Synonyms Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); T200; PTPRC
Location Cell membrane
Accession P08575
Clone Number S-R401
Antibody Type Mouse mAb
Isotype IgG2a,k
Application WB, IHC-P, ICC, FCM, IF
Reactivity Hu
Purification Protein A
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000
IHC-P 1:1000
ICC 1:500
IF 1:200
FCM 1:2000

Background

CD45RO is an important cell surface marker in immunology that belongs to the CD45 family (also known as Leukocyte Common Antigen, LCA). CD45 is a highly polymorphic transmembrane protein primarily expressed on the surface of white blood cells, including T cells, B cells, natural killer (NK) cells, monocytes, and granulocytes, and plays a crucial role in lymphocyte development, activation, and signaling. Specifically, CD45RO is an isoform of CD45 that is highly expressed on memory T cells but expressed at lower levels or not expressed on naive T cells. Memory T cells are T cells that have been activated and survived during previous immune responses, enabling them to rapidly and strongly activate upon re-encountering the same antigen, thus accelerating and enhancing immune responses. Therefore, CD45RO is often used to distinguish and identify memory T cells. It serves as an important marker in immune phenotyping analysis, disease diagnosis, immune response monitoring, and immunotherapy research.

Picture

Western Blot

WB result of CD45RO Mouse mAb
Primary antibody: CD45RO Mouse mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HuT 78 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 180 kDa
Observed MW: 180 kDa

FC

Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, left) / HuT 78 (Human Sezary syndrome cutaneous T lymphocyte, Right) labelling Human CD45RO antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: Jurkat

Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD45RO antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45RA - PE/Cy5.5 separately. Gated on viable lymphocytes.

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human spleen. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded non-Hodgkin’s T-cell lymphoma. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human anaplastic large cell lymphoma. Anti-CD45RO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HuT 78 cells (top panel) and negative staining in Jurkat cells (below panel). Anti-CD45RO antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

Immunofluorescence

IF shows positive staining in paraffin-embedded human tonsil. Anti-CD45RO antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded human anaplastic large cell lymphoma. Anti-CD45RO antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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