Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD35 antibody at 1/50 (1 μg) dilution (Right) compared with a Rabbit monoclonal IgG isotype control (Left). Goat Anti - Rabbit IgG DyLight® 488 was used as the secondary antibody. Then cells were stained with CD19 - Alexa Fluor® 647 separately. Gated on total viable cells.
CD35 Recombinant Rabbit mAb (S-1294-26)
CD35 Recombinant Rabbit mAb (S-1294-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD35 |
| Synonyms | Complement receptor type 1, C3b/C4b receptor, CR1, C3BR |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | P17927 |
| Clone Number | S-1294-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:50 | null |
Background
CD35 is a type I single chain of glycoprotein, also known as C3b/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, follicular dendritic cells and renal foot cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. In pathology, it is mainly used in the diagnosis of follicular dendritic cell tumor.
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