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CD25/IL-2Rα Recombinant Rabbit mAb (S-548-137)

CD25/IL-2Rα Recombinant Rabbit mAb (S-548-137)

Catalog Number: S0B0704 Application: WB,ICC Reactivity: Mouse Conjugation: Unconjugated Brand: Starter
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Regular price $130.00 SGD
Regular price Sale price $130.00 SGD
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Product Details

Product Specification


Host Rabbit
Antigen CD25/IL-2Rα
Synonyms Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, Il2ra, Il2r
Immunogen Synthetic Peptide
Location Membrane
Accession P01590
Clone Number S-548-137
Antibody Type Recombinant mAb
Isotype IgG
Application WB, ICC
Reactivity Ms
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20°C as supplied

Dilution


application dilution species
WB 1:1000 null
ICC 1:500 null

Background

CD25, also known as the interleukin-2 receptor alpha chain (IL-2Rα), is a protein that serves as a receptor for interleukin-2 (IL-2), a cytokine that plays a crucial role in immune system regulation. CD25 is expressed on the surface of various immune cells, including T cells, B cells, natural killer (NK) cells, dendritic cells, and monocytes. The main function of CD25 is to bind to IL-2, which then triggers a signaling cascade that leads to the activation, proliferation, and differentiation of immune cells. CD25 expression is tightly regulated and is often used as a marker for regulatory T cells (Tregs), a subset of T cells that play a key role in suppressing immune responses and maintaining immune homeostasis. Tregs are characterized by high expression levels of CD25 and the transcription factor FoxP3. Abnormalities in CD25 expression or function have been linked to various immunological disorders. For example, deficiencies in CD25 expression or function can lead to immunodeficiency, while excessive CD25 expression has been associated with autoimmune diseases and cancer.

Picture

Western Blot

WB result of CD25/IL-2Rα Rabbit mAb
Primary antibody: CD25/IL-2Rα Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: CTLL-2 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate; C2C12 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 35~70 kDa

Immunocytochemistry

ICC shows positive staining in CTLL-2 cells (top panel) and negative staining in C2C12 cells (below panel). Anti- CD25/IL-2Rα antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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