Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast, left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD21 (Alexa Fluor® 594 Conjugate) antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Negative control: K562
CD21 Mouse mAb (Alexa Fluor® 594 Conjugate) (S-764-18)
CD21 Mouse mAb (Alexa Fluor® 594 Conjugate) (S-764-18)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD21 |
| Synonyms | Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor (EBV receptor) |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P20023 |
| Clone Number | S-764-18 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | ICC, FCM |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 594 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:2000 | |
| ICC | 1:200 |
Background
CD21 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d.B cells express CD21 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation. CD21 interacts with CD19 and on mature B cells forms a complex with CD81 (TAPA-1). The CD21-CD19-CD81 complex is often called the B cell co-receptor complex, because CD21 binds to opsonized antigens through attached C3d (or iC3b or C3dg) when the B-cell receptor binds antigen. This results in the B cell having greatly enhanced response to the antigen. Epstein-Barr virus (EBV) can bind CD21, enabling EBV to enter and infect B cells.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in Raji cells. Anti- CD21 (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control：ICC shows negative staining in K562 cells. Anti- CD21 (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
