WB result of CD1a Recombinant Rabbit mAb
Primary antibody: CD1a Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa
Observed MW: 42 kDa
CD1a Recombinant Rabbit mAb (S-1486-65)
CD1a Recombinant Rabbit mAb (S-1486-65)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD1a |
| Synonyms | T-cell surface glycoprotein CD1a; T-cell surface antigen T6/Leu-6 (hTa1 thymocyte antigen) |
| Immunogen | Recombinant Protein |
| Accession | P06126 |
| Clone Number | S-1486-65 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | Molt-4, Jurkat |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Background
CD1a is a member of the CD1 family of cell-surface glycoproteins that present lipid antigens to T cells. It is structurally related to MHC class I molecules, but its antigen-binding cleft is specifically designed to accommodate lipids rather than peptides. CD1a is characterized by its small antigen-binding groove and high expression on Langerhans cells in the epidermis. CD1a is involved in the adaptive immune response to many microbial lipid antigens. It is expressed on immature thymocytes and is lost upon T cell maturation. In the skin, CD1a is constitutively highly expressed on Langerhans cells, which are ideally positioned to detect breaches in the skin barrier and changes in the local extracellular milieu. Beyond Langerhans cells, CD1a is expressed at lower levels on a subset of dermal myeloid dendritic cells. In inflammatory skin diseases such as atopic dermatitis and psoriasis, the frequency of CD1a-expressing dendritic cell subsets is increased, and migratory patterns of Langerhans cells are altered.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human esophagus. Anti-CD1a antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD1a antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CD1a antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
