Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD19 (Cy5 Conjugate) antibody at 1/130 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Negative control: Jurkat
CD19 Recombinant Rabbit mAb (Cy5 Conjugate) (S-164-41)
CD19 Recombinant Rabbit mAb (Cy5 Conjugate) (S-164-41)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD19 |
| Synonyms | B-lymphocyte antigen CD19, LE-CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P15391 |
| Clone Number | S-164-41 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 1.3 mg/ml |
| Conjugation | Cy5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500 | |
| FCM | 1:130 |
Background
The CD19 antigen plays an important role in clinical oncology. In normal cells, it is the most ubiquitously expressed protein in the B lymphocyte lineage. CD19 expression is induced at the point of B lineage commitment during the differentiation of the hematopoietic stem cell, and its expression continues through preB and mature B cell differentiation until it is finally down-regulated during terminal differentiation into plasma cells. CD19 expression is maintained in B-lineage cells that have undergone neoplastic transformation, and therefore CD19 is useful in diagnosis of leukemias and lymphomas using monoclonal antibodies (mAbs) and flow cytometry [PMID: 8528044].
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in Raji cells. Anti-CD19 (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in Jurkat cells. Anti-CD19 (Cy5 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
