Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cells) labelling Human CD138 Antibody antibody at 1/50 (1 μg) dilution/ (Right panel) compared with a Rabbit IgG Isotype Control / (Left panel). Goat Anti - Rabbit lgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ antibody separately.
CD138 Recombinant Rabbit mAb (S-417-199)
CD138 Recombinant Rabbit mAb (S-417-199)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD138 |
| Synonyms | Syndecan-1; SYND1; SDC1 |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | P18827 |
| Clone Number | S-417-199 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, ICC, FCM |
| Reactivity | Hu |
| Positive Sample | U266B1 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:200 | Hu |
| ICC | 1:500 | Hu |
| FCM | 1:50 | Hu |
Background
Syndecan-1 (CD138) is mostly restricted to epithelia, and bears heparan sulfate chains that are capable of interacting with a large array of polypeptides, including extracellular matrix components and potent mediators of proliferation, adhesion and migration. For this reason, it has been studied extensively with respect to carcinomas and tumor progression. Frequently, but not always, syndecan-1 levels decrease as tumor grade, stage and invasiveness and dedifferentiation increase. However, in some tumors, levels of syndecan-1 increase, but the characterization of its distribution is relevant. There can be loss of membrane staining, but acquisition of cytoplasmic and/or nuclear staining that is abnormal. Moreover, the appearance of syndecan-1 in the tumor stroma, either associated with its cellular component or the collagenous matrix, is nearly always a sign of poor prognosis.
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Picture
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-CD138 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD138 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD138 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human transitional cell carcinoma. Anti-CD138 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in U266B1 cells (top panel) and negative staining in Raji cells (below panel). Anti-CD138 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
