WB result of CD123 Recombinant Rabbit mAb
Primary antibody: CD123 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Negative control: Raji whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 75 kDa
CD123 Recombinant Rabbit mAb (S-1592-12)
CD123 Recombinant Rabbit mAb (S-1592-12)
Price:
Regular price
$130.00 SGD
Regular price
Sale price
$130.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD123 |
| Synonyms | Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; IL3RA; IL3R |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P26951 |
| Clone Number | S-1592-12 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | THP-1 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:100 | Hu |
Background
CD123, also known as the interleukin-3 receptor alpha chain (IL-3Rα), is a glycoprotein composed of 360 amino acids. It forms a high-affinity IL-3 receptor with CD131, and by binding to the IL-3 ligand, it promotes the proliferation and survival of hematopoietic stem/progenitor cells (HSPCs). In various hematological malignancies, such as acute myeloid leukemia (AML), blastic plasmacytoid dendritic cell neoplasm (BPDCN), acute lymphoblastic leukemia/lymphoma (ALL), hairy cell leukemia (HCL), and systemic mastocytosis (SM), the expression level of CD123 is often high and associated with the overproliferation of tumor cells. It is worth noting that the expression level of CD123 increases with the progression and worsening of hematological malignancies, indicating that the expression level of CD123 may be related to the severity of the disease. Moreover, high expression of CD123 is associated with poor prognosis in AML, making it an ideal target for AML immunotherapy.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD123 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD123 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenomcarcinoma. Anti-CD123 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
