WB result of CD112/Nectin-2 Recombinant Rabbit mAb
Primary antibody: CD112/Nectin-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 70 kDa
CD112/Nectin-2 Recombinant Rabbit mAb (S-2404-66)
CD112/Nectin-2 Recombinant Rabbit mAb (S-2404-66)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD112/Nectin-2 |
| Synonyms | Nectin-2; Herpes virus entry mediator B (Herpesvirus entry mediator B; HveB); Nectin cell adhesion molecule 2; Poliovirus receptor-related protein 2; HVEB; PRR2; PVRL2; NECTIN2 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q92692 |
| Clone Number | S-2404-66 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, SH-SY5Y, 293T, NIH/3T3, Neruo-2a |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu |
Background
CD112, also known as PRR2 or Nectin-2, is a 58 kDa protein expressed in myeloid cells. It is a component of adherens junctions and plays a role in cell adhesion. CD112 is a member of the nectin family, which are calcium-independent immunoglobulin superfamily proteins that mediate cell-cell adhesion through homophilic and heterophilic interactions. Nectins are involved in the formation and maintenance of adherens junctions and tight junctions, and they regulate various cellular behaviors such as cell adhesion, growth, differentiation, migration, and apoptosis.
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Picture
Western Blot
WB result of CD112/Nectin-2 Recombinant Rabbit mAb
Primary antibody: CD112/Nectin-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 70 kDa
WB result of CD112/Nectin-2 Recombinant Rabbit mAb
Primary antibody: CD112/Nectin-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-CD112/Nectin-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-CD112/Nectin-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-CD112/Nectin-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CD112/Nectin-2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
