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CD105 Recombinant Rabbit mAb (S-3764-11)

CD105 Recombinant Rabbit mAb (S-3764-11)

Catalog Number: S0B6763 Application: WB, IHC-P Reactivity: Mouse, Rat Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen CD105
Synonyms Endoglin; Cell surface MJ7/18 antigen; Edg; Eng
Immunogen Synthetic Peptide
Location Cell membrane
Accession Q63961
Clone Number S-3764-11
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Ms, Rt
Positive Sample bEnd.3
Purification Protein A
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:500-1:1000 Ms, Rt
IHC-P 1:2000 Ms, Rt

Background

CD105, also known as Endoglin, is a type I transmembrane glycoprotein and a component of the transforming growth factor-beta (TGF-β) receptor complex. It is highly expressed in proliferating endothelial cells and plays a crucial role in angiogenesis and vascular development. CD105 is upregulated in areas of active vascular growth, such as tumor tissues, wound healing sites, and inflamed tissues, making it a valuable marker for studying tumor-related angiogenesis. Additionally, it is involved in maintaining the integrity of the vascular system and regulating immune responses. In clinical applications, CD105 is used as a diagnostic marker for certain cancers and vascular diseases, and it is also a potential target for anti-angiogenic therapies.

Picture

Western Blot

WB result of CD105 Recombinant Rabbit mAb
Primary antibody: CD105 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: bEnd.3 whole cell lysate 20 µg 
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 85 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CD105 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CD105 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-CD105 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse lung. Anti-CD105 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-CD105 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat lung. Anti-CD105 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD105 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.